pGLO Observations , Data Recording & Analysis
1.
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Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
Comments: The -pGLO LB and the -pGLO LB/amp plates were switched when labeled.
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2.
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What two new traits do your transformed bacteria have?
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The resistance to the ampicillin and the bacteria could glow under UV light.
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3.
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Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
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If you use the carpet for reference, I think that there are about a thousand to ten thousand bacteria. The reason that I think this is because the carpet is very spread out along the plate, so there will be a lot of bacteria.
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4.
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What is the role of arabinose in the plates?
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The arabinose, which is a sugar, had the effect of making the bacteria glow under the UV light. That is why, when you look at the plate with the arabinose with the ampicillin, LB and the pGLO and compare it to the exact same plate without the arabinose, you see that the one with arabinose will glow.
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5.
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List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
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The GFP is used because of its ability to generate a glowing color. GFP is used as a active indicator for protease action because then the scientists would know that it is working. Another reason why GFP is used is because it can glow inside an organism so the scientists could see what goes on inside the
organisms cell. The third use is GFP is used for Biosensors, which allows you to analyze different conditions, like pH levels. | ||
6.
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Give an example of another application of genetic engineering.
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In medicine, genetic engineering has been used to mass produce human growth hormones, insulin, monoclonal antibodies, vaccines and many more.
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